TT-seq maps the human transient transcriptome.
Science
; 352(6290): 1225-8, 2016 Jun 03.
Article
em En
| MEDLINE
| ID: mdl-27257258
ABSTRACT
Pervasive transcription of the genome produces both stable and transient RNAs. We developed transient transcriptome sequencing (TT-seq), a protocol that uniformly maps the entire range of RNA-producing units and estimates rates of RNA synthesis and degradation. Application of TT-seq to human K562 cells recovers stable messenger RNAs and long intergenic noncoding RNAs and additionally maps transient enhancer, antisense, and promoter-associated RNAs. TT-seq analysis shows that enhancer RNAs are short-lived and lack U1 motifs and secondary structure. TT-seq also maps transient RNA downstream of polyadenylation sites and uncovers sites of transcription termination; we found, on average, four transcription termination sites, distributed in a window with a median width of ~3300 base pairs. Termination sites coincide with a DNA motif associated with pausing of RNA polymerase before its release from the genome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Polimerases Dirigidas por DNA
/
RNA Mensageiro
/
Regiões Terminadoras Genéticas
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Transcriptoma
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Terminação da Transcrição Genética
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article