A gradient-free method for the purification of infective dengue virus for protein-level investigations.
J Virol Methods
; 235: 125-130, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27265428
ABSTRACT
Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Virologia
/
Vírus da Dengue
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article