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Exogenous l-arginine reduces matrix metalloproteinase-2 and -9 activities and oxidative stress in patients with hypertension.
Garcia, Vinicius P; Rocha, Helena N M; Silva, Gustavo M; Amaral, Tatiana A G; Secher, Niels H; Nóbrega, Antonio C L; Vianna, Lauro C; Rocha, Natália G.
Afiliação
  • Garcia VP; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil.
  • Rocha HNM; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil.
  • Silva GM; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil.
  • Amaral TAG; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil.
  • Secher NH; Department of Anaesthesiology, The Copenhagen Muscle Research Center, Rigshospitalet, University of Copenhagen, Denmark.
  • Nóbrega ACL; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil.
  • Vianna LC; Faculty of Physical Education, University of Brasília, Brasília, Brazil.
  • Rocha NG; Laboratory of Exercise Sciences, Department of Physiology and Pharmacology, Fluminense Federal University, Niterói, Brazil. Electronic address: nataliagalito@id.uff.br.
Life Sci ; 157: 125-130, 2016 Jul 15.
Article em En | MEDLINE | ID: mdl-27287681
AIMS: Increased matrix metalloproteinases activity and reduced nitric oxide (NO) bioavailability contributes to development of hypertension and this may be associated with a defective l-arginine-NO pathway. Exogenous l-arginine improves endothelial function to prevent the onset of cardiovascular disease, but the mechanism by which this is accomplished remains unclear. We determined the effects of exogenous l-arginine infusion on vascular biomarkers in patients with hypertension. MAIN METHODS: Venous blood samples were obtained from seven patients with hypertension (45±5yrs., HT group) and eleven normotensive men (37±3yrs., CT group) before and during a 30-min intravenous l-arginine or saline infusion. Nitrite concentration was evaluated by ozone-chemiluminescence method; metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) activities were detected by zymography; tissue inhibitor of metalloproteinases-1 (TIMP-1) and 8-isoprostane concentrations were measured by enzyme-linked immunosorbent assay (ELISA); and thiobarbituric acid reactive substances (TBARS) were determined by colorimetric assay. KEY FINDINGS: At baseline, nitrite, TIMP-1, and MMP-2 activity were similar between the groups (P>0.05), but MMP-9, TBARS and 8-isoprostane were higher in HT group (P≤0.03). During l-arginine infusion, nitrite increased only in control group (P=0.01), while MMP-2, MMP-9 activities, MMP-9/TIMP-1 ratio and 8-isoprostane decreased in HT group (P≤0.02). There were no significant changes in vascular biomarkers between groups during the saline infusion (P>0.05). SIGNIFICANCE: Exogenous l-arginine diminished metalloproteinase-2 and -9 activities and MMP-9/TIMP-1 ratio along with restoring the oxidative stress balance in patients with hypertension.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginina / Estresse Oxidativo / Metaloproteinase 2 da Matriz / Metaloproteinase 9 da Matriz / Hipertensão Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arginina / Estresse Oxidativo / Metaloproteinase 2 da Matriz / Metaloproteinase 9 da Matriz / Hipertensão Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article