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[Proteomic Analysis of Rat Brain Stem with DAI by MALDI-TOF-MS].
Fa Yi Xue Za Zhi ; 32(1): 13-7, 2016 Feb.
Article em Zh | MEDLINE | ID: mdl-27295850
OBJECTIVE: To establish a diagnostic model for diffuse axonal injury (DAI) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). To screen the proteins or peptides associated with DAI for providing the biomarkers with theoretic foundation. METHODS: Fifteen male Sprague-Dawley rats were randomly divided into DAI group (n = 10) and control group (n = 5). The protein or peptide expression profiles of rat brain stem were detected by MALDI-TOF-MS. ClinProTools 2.2 software was used to find specific peaks, and a diagnostic model was established by the genetic algorithm. RESULTS: There were significant differences in 61 peaks of DAI group (P < 0.05), 9 peaks were down-regulated and 52 up-regulated. The diagnostic model was established based on 5 different peaks. The specificity and sensitivity of cross validation was 96.14% and 95.98%; while the specificity and sensitivity of blind validation showed was 73.33% and 70.00%, respectively. CONCLUSION: A specific and sensitive diagnostic model of DAI can be established by MALDI-TOF-MS to provide a potential value for determining DAI in forensic practice.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Tronco Encefálico / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Lesão Axonal Difusa / Proteômica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2016 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Tronco Encefálico / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Lesão Axonal Difusa / Proteômica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2016 Tipo de documento: Article