Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803.
Metab Eng
; 38: 56-64, 2016 11.
Article
em En
| MEDLINE
| ID: mdl-27328433
ABSTRACT
Synechocystis PCC 6803 is a model unicellular cyanobacterium used in e.g. photosynthesis and CO2 assimilation research. In the present study we examined the effects of overexpressing Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), sedoheptulose 1,7-biphosphatase (SBPase), fructose-bisphosphate aldolase (FBA) and transketolase (TK), confirmed carbon flux control enzymes of the Calvin-Bassham-Benson (CBB) cycle in higher plants, in Synechocystis PCC 6803. Overexpressing RuBisCO, SBPase and FBA resulted in increased in vivo oxygen evolution (maximal 115%), growth rate and biomass accumulation (maximal 52%) under 100µmolphotonsm-2s-1 light condition. Cells overexpressing TK showed a chlorotic phenotype but increased biomass by approximately 42% under 100µmolphotonsm-2s-1 light condition. Under 15µmolphotonsm-2s-1 light condition, cells overexpressing TK showed enhanced in vivo oxygen evolution. This study demonstrates increased growth and biomass accumulation when overexpressing selected enzymes of the CBB cycle. RuBisCO, SBPase, FBA and TK are identified as four potential targets to improve growth and subsequently also yield of valuable products from Synechocystis PCC 6803.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fotossíntese
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Carbono
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Synechocystis
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Enzimas
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Redes e Vias Metabólicas
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Engenharia Metabólica
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article