Independent catalysis of the short form HisG from Lactococcus lactis.
FEBS Lett
; 590(16): 2603-10, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27393206
ABSTRACT
ATP-phosphoribosyltransferase (ATP-PRT) catalyses the first step of histidine biosynthesis. Two different forms of ATP-PRT have been described; the homo-hexameric long form, and the hetero-octameric short form. Lactococcus lactis possesses the short form ATP-PRT comprising four subunits of HisGS , the catalytic subunit, and four subunits of HisZ, a histidyl-tRNA synthetase paralogue. Previous studies have suggested that HisGS requires HisZ for catalysis. Here, we reveal that the dimeric HisGS does display ATP-PRT activity in the absence of HisZ. This result reflects the evolutionary relationship between the long and short form ATP-PRT, which acquired allosteric inhibition and enhanced catalysis via two divergent strategies.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
ATP Fosforribosiltransferase
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Proteínas de Bactérias
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Proteínas de Transporte de Monossacarídeos
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Isoformas de Proteínas
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Aminoacil-tRNA Sintetases
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article