Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes.
Biochem Biophys Res Commun
; 478(1): 343-348, 2016 09 09.
Article
em En
| MEDLINE
| ID: mdl-27416753
ABSTRACT
Transglutaminase (TGase) catalyzes protein cross-linking reactions essential for several biological processes. In differentiating keratinocytes, TG1 (keratinocyte-type) is crucial for the cross-linking of substrate proteins required for the complete formation of the cornified envelop, a proteinaceous supermolecule located in the outermost layer of the epidermis. TG1 expressions and its substrate were induced in cultured keratinocytes at differentiation-stage specific manner. In the cultured keratinocytes, we used the TG1-specific substrate peptide, which enables the specific detection of enzymatic activity to investigate its induction patterns. As a further application of the substrate peptide, several substrate candidates of TG1 that may be essential for cornified envelope formation were identified and characterized.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Regulação Enzimológica da Expressão Gênica
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Queratinócitos
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Transglutaminases
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article