LEVER: software tools for segmentation, tracking and lineaging of proliferating cells.
Bioinformatics
; 32(22): 3530-3531, 2016 11 15.
Article
em En
| MEDLINE
| ID: mdl-27423896
ABSTRACT
The analysis of time-lapse images showing cells dividing to produce clones of related cells is an important application in biological microscopy. Imaging at the temporal resolution required to establish accurate tracking for vertebrate stem or cancer cells often requires the use of transmitted light or phase-contrast microscopy. Processing these images requires automated segmentation, tracking and lineaging algorithms. There is also a need for any errors in the automated processing to be easily identified and quickly corrected. We have developed LEVER, an open source software tool that combines the automated image analysis for phase-contrast microscopy movies with an easy-to-use interface for validating the results and correcting any errors. AVAILABILITY AND IMPLEMENTATION LEVER is available free and open source, licensed under the GNU GPLv3. Details on obtaining and using LEVER are available at http//n2t.net/ark/87918/d9rp4t CONTACT acohen@coe.drexel.edu.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
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Linhagem da Célula
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Proliferação de Células
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article