Interaction properties of human TRAMP-like proteins and their role in pre-rRNA 5'ETS turnover.
FEBS Lett
; 590(17): 2963-72, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27434818
ABSTRACT
In yeast, the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) complex acts as a cofactor for the nuclear exosome to promote degradation of various RNAs. However, the corresponding machinery in mammals is less characterized. We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly. Furthermore, the human TRAMP-like proteins were involved in the RRP6-catalyzed turnover of pre-rRNA 5'ETS fragments. These results suggest the significant role for RRP6 in the assembly of TRAMP-like proteins during nucleolar RNA surveillance.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Nucleotidiltransferases
/
Fatores de Transcrição
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RNA Helicases
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Estabilidade de RNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article