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Effect of confounding factors on a phospho-flow assay of ribosomal S6 protein for therapeutic drug monitoring of the mTOR-inhibitor everolimus in heart transplanted patients.
Dieterlen, Maja-Theresa; John, Katja; Haase, Susanne; Garbade, Jens; Tarnok, Attila; Mohr, Friedrich W; Bittner, Hartmuth B; Barten, Markus J.
Afiliação
  • Dieterlen MT; a HELIOS Clinic, Department of Cardiac Surgery , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • John K; a HELIOS Clinic, Department of Cardiac Surgery , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • Haase S; a HELIOS Clinic, Department of Cardiac Surgery , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • Garbade J; a HELIOS Clinic, Department of Cardiac Surgery , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • Tarnok A; b HELIOS Clinic, Department of Pediatric Cardiology , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • Mohr FW; a HELIOS Clinic, Department of Cardiac Surgery , University Hospital Leipzig, Heart Center , Leipzig , Germany.
  • Bittner HB; c Department of Cardiothoracic Transplantation and Advanced Cardiac Surgery , Florida Hospital Orlando , Orlando , FL , USA.
  • Barten MJ; d Department of Cardiovascular Surgery , University Heart Center Hamburg , Hamburg , Germany.
Biomarkers ; 22(1): 86-92, 2017 Feb.
Article em En | MEDLINE | ID: mdl-27448205
ABSTRACT
CONTEXT Several assays of monitoring immune cell function have been developed to enhance therapeutic drug monitoring.

OBJECTIVE:

An in vitro-validated whole-blood assay of phosphorylated ribosomal protein S6 (pS6RP) was evaluated for confounders to monitor the mTOR-inhibitor everolimus (ERL). MATERIALS AND

METHODS:

Whole blood samples from 87 heart transplant recipients were analyzed for pS6RP-expression in CD3-positive T-cells by phospho-flow analysis.

RESULTS:

ERL blood concentration, laboratory parameters, co-medications, demographic and clinical data were reviewed.

CONCLUSION:

Evaluating the pS6RP-assay revealed that pS6RP is influenced by cyclosporine A (CsA) blood concentration, duration of ERL treatment, co-medication with thiazide diuretics and different metabolic parameters.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Coração / Proteína S6 Ribossômica / Everolimo Limite: Humans / Middle aged Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Coração / Proteína S6 Ribossômica / Everolimo Limite: Humans / Middle aged Idioma: En Ano de publicação: 2017 Tipo de documento: Article