The RTA3 Gene, Encoding a Putative Lipid Translocase, Influences the Susceptibility of Candida albicans to Fluconazole.
Antimicrob Agents Chemother
; 60(10): 6060-6, 2016 10.
Article
em En
| MEDLINE
| ID: mdl-27480868
ABSTRACT
The RTA3 gene, coding for a member of the Rta1p-like lipid-translocating exporter family, is coordinately upregulated with the ATP-binding cassette transporter genes CDR1 and CDR2 in azole-resistant clinical isolates of Candida albicans that carry activating mutations in the transcription factor Tac1p. We show here that deleting RTA3 in an azole-resistant clinical isolate carrying a Tac1p-activating mutation lowered fluconazole resistance by 2-fold, while overexpressing RTA3 in an azole-susceptible clinical isolate resulted in enhanced fluconazole tolerance associated with trailing growth in a liquid microtiter plate assay. We also demonstrate that an Rta3p-green fluorescent protein (GFP) fusion protein localizes predominantly to the plasma membrane, consistent with a putative function for Rta3p as a lipid translocase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
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Proteínas Fúngicas
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Fluconazol
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Regulação Fúngica da Expressão Gênica
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Farmacorresistência Fúngica
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Proteínas de Transferência de Fosfolipídeos
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Antifúngicos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article