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A genetic assay for gene essentiality in Clostridium.
Walker, David J F; Heap, John T; Winzer, Klaus; Minton, Nigel P.
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  • Walker DJF; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
  • Heap JT; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
  • Winzer K; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
  • Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom; Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The Universi
Anaerobe ; 42: 40-43, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27487328
Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triptofano-tRNA Ligase / Bioensaio / Engenharia Genética / Clostridioides difficile / Genes Essenciais / Metionina Adenosiltransferase Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triptofano-tRNA Ligase / Bioensaio / Engenharia Genética / Clostridioides difficile / Genes Essenciais / Metionina Adenosiltransferase Idioma: En Ano de publicação: 2016 Tipo de documento: Article