An Infectious Pig Model of Crohn's Disease.

ABSTRACT

BACKGROUND: Over the last several years, we have demonstrated that intestinal lymphangitis and lymphatic obstruction are fundamental lesions in Crohn's disease, for which no therapy is currently available. There is an infectious enteritis of young pigs that offers an opportunity to understand how the lymphangitis of CD may have been initiated. The pathology of chlamydial enteritis was described earlier, from 1987 to 2009. MATERIALS AND METHODS: Tissue blocks and hematoxylin and eosin-stained slides from Chlamydia suis-inoculated young pigs were provided by D. Rogers and F. Guscetti. Experimental animals, gnotobiotic and conventional, had been autopsied 4, 7, and 10 days postinoculation. Serial sections of intestine were immunostained with a Chlamydia trachomatis antibody, which cross-reacted with C. suis antigen, and compared with hematoxylin and eosin preparations. RESULTS: Immunohistochemistry revealed antigen in villous epithelial cells of jejunum and ileum and in the endothelium of lacteals and lymphatics by day 4. This was accompanied by lymphatic endothelial necrosis, lymphangitis, and inflammatory lymphatic obstruction, through several layers of the affected intestinal segments, days 4 through 10. CONCLUSIONS: Although the original authors documented lesions to define the porcine disease, here the author characterizes the lymphangitis as a model for understanding Crohn's disease and suggests a chlamydial origin for the latter.