Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates.
Anal Bioanal Chem
; 408(27): 7559-7563, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-27544520
ABSTRACT
A novel washing technique for microfluidic paper-based analytical devices (µPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported. Liquids can flow through a porous medium (such as paper) in the absence of external pressure as a result of capillary action. Uniform results were achieved when washing a paper substrate in a PDMS holder which was integrated with a cartridge absorber acting as a porous medium. Our study demonstrated that applying this washing technique would allow µPADs to become the least expensive microfluidic device platform with high reproducibility and sensitivity. In a model µPAD assay that utilized this novel washing technique, C-reactive protein (CRP) was detected with a limit of detection (LOD) of 5 µg mL-1. Graphical Abstract A novel washing technique for microfluidic paper-based analytical devices (µPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína C-Reativa
/
Imunoensaio
/
Técnicas Analíticas Microfluídicas
/
Dispositivos Lab-On-A-Chip
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article