Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.
Zebrafish
; 14(1): 73-76, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27548568
ABSTRACT
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
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Peixe-Zebra
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Animais Geneticamente Modificados
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Sistema Livre de Células
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Proteínas de Peixe-Zebra
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Técnicas de Inativação de Genes
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article