Hydrogen peroxide derived from marine peroxy sesquiterpenoids induces apoptosis in HCT116 human colon cancer cells.
Bioorg Med Chem Lett
; 26(19): 4641-4644, 2016 10 01.
Article
em En
| MEDLINE
| ID: mdl-27575468
ABSTRACT
In this study, the isolates of the peroxy sesquiterpenoids (1-3) from the Okinawan soft coral, Sinularia sp., indicated cytotoxicity in HCT116 colon cancer cells. The apoptotic cells with a nuclear condensation were detected in the presence of these compounds, then the caspase 3/7 activity was induced, indicating that the compounds have a potential antitumor activity by apoptosis-induction. The cells treated with these compounds were generated reactive oxygen species (ROS), indicating that the ROS is related to the induction of apoptosis. The ROS production reduced in the presence of catalase or trolox, indicating that hydrogen peroxide (H2O2) is generated through a certain free radical reaction derived from the compound. In fact, the accumulation of intracellular H2O2 was also confirmed in the presence of these compounds. Based on all the results, this study proposed the apoptosis-inducing mechanism due to the compounds that the H2O2 produced involving free radical reactions derived from cleavage of the end or hydro-peroxide in the molecule induced cell death.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
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Apoptose
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Neoplasias do Colo
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Peróxido de Hidrogênio
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article