Comparation of the effects of different 5'-untranslated regions (UTRs) on gene expression in HEK293 cells.
Biotechnol Lett
; 38(12): 2051-2057, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27580891
ABSTRACT
OBJECTIVES:
To evaluate four 5'-UTRs on GFP expression in HEK293T cells.RESULTS:
The recombinant plasmids were constructed by restriction enzyme digestion, digestion and DNA sequencing. Quantitative real-time PCR and western blotting results showed that the transcription and translation level of PPRV-GFP mRNA was significantly lower than that of the other reporters. The transcription and translation level of ChEF1-GFP was the highest in HEK293T cells.CONCLUSIONS:
Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.Palavras-chave
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Regiões 5' não Traduzidas
/
Vetores Genéticos
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article