Comparation of the effects of different 5'-untranslated regions (UTRs) on gene expression in HEK293 cells.

ABSTRACT

OBJECTIVES: To evaluate four 5'-UTRs on GFP expression in HEK293T cells. RESULTS: The recombinant plasmids were constructed by restriction enzyme digestion, digestion and DNA sequencing. Quantitative real-time PCR and western blotting results showed that the transcription and translation level of PPRV-GFP mRNA was significantly lower than that of the other reporters. The transcription and translation level of ChEF1-GFP was the highest in HEK293T cells. CONCLUSIONS: Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.