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High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML.
Ma, Leyuan; Roderick, Justine; Kelliher, Michelle A; Green, Michael R.
Afiliação
  • Ma L; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA. leyuan.ma@umassmed.edu.
  • Roderick J; Howard Hughes Medical Institute, Chevy Chase, MD, USA. leyuan.ma@umassmed.edu.
  • Kelliher MA; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA.
  • Green MR; Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA.
Methods Mol Biol ; 1465: 159-73, 2016.
Article em En | MEDLINE | ID: mdl-27581147
ABSTRACT
Genome-wide RNA interference (RNAi) screening in mammalian cells has proven to be a powerful tool for identifying new genes and molecular pathways relevant to many cellular processes and diseases. For example, screening for genes that, when inactivated, lead to resistance to cancer therapeutic drugs can reveal new mechanisms for how resistance develops and identify potential targetable strategies to overcome drug resistance. Here, we describe a detailed procedure for performing a high-throughput RNAi screen using a genome-wide human short hairpin RNA (shRNA) library for identifying tyrosine kinase inhibitor (TKI)-resistance genes in a human CML cell line model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucemia Mielogênica Crônica BCR-ABL Positiva / Resistencia a Medicamentos Antineoplásicos / RNA Interferente Pequeno / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucemia Mielogênica Crônica BCR-ABL Positiva / Resistencia a Medicamentos Antineoplásicos / RNA Interferente Pequeno / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article