Automated nanoscale flow cytometry for assessing protein-protein interactions.
Cytometry A
; 89(9): 835-43, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27584593
ABSTRACT
Despite their importance for signalling events, protein-protein interactions cannot easily be analyzed on a single cell level. We developed a robust automated FRET measurement system implemented on a commercial flow cytometer allowing for rapid profiling of molecular associations in living cells. We used this method to measure the most proximal signaling events on human T lymphocyte activation, which preceded calcium influx, and could automatically detect T cell receptor/CD3 complex clustering defects in immunocompromised patients. © 2016 International Society for Advancement of Cytometry.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Interação de Proteínas
/
Análise de Célula Única
/
Mapas de Interação de Proteínas
/
Citometria de Fluxo
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article