Your browser doesn't support javascript.

Portal de Pesquisa da BVS

Informação e Conhecimento para a Saúde

Home > Pesquisa > ()
XML
Imprimir Exportar

Formato de exportação:

Exportar

Email
Adicionar mais destinatários
| |

Determination of Fetal RHD Genotype Including the RHD Pseudogene in Maternal Plasma.

Ziza, Karen Chinoca; Liao, Adolfo Wenjaw; Dezan, Marcia; Dinardo, Carla Luana; Jens, Eduardo; Francisco, Rossana Pulcineli Vieira; Junior, Alfredo Mendrone; Zugaib, Marcelo; Levi, José Eduardo.
J Clin Lab Anal ; 31(3)2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27595845

OBJECTIVE:

To examine the accuracy of fetal RHD genotype and RHD pseudogene determination in a multiethnical population.

METHODS:

Prospective study involving D-negative pregnant women. Cell-free DNA was extracted from 1 ml of maternal plasma by an automated system (MagNA Pure Compact, Roche) and real-time PCR was performed in triplicate targeting the RHD gene exons 5 and 7. Inconclusive samples underwent RHD pseudogene testing by real-time PCR analysis employing novel primers and probe.

RESULTS:

A positive result was observed in 128/185 (69.2%) samples and negative in 50 (27.0%). Umbilical cord blood phenotype confirmed all cases with a positive or negative PCR result. Seven (3.8%) cases were found inconclusive (exon 7 amplification only) and RHD pseudogene testing with both conventional and real-time PCR demonstrated a positive result in five of them, while two samples were also RHD pseudogene negative.

CONCLUSION:

Real-time PCR targeting RHD exons 5 and 7 simultaneously in maternal plasma is an accurate method for the diagnosis of fetal D genotype in our population. The RHD pseudogene real-time PCR assay is feasible and is particularly useful in populations with a high prevalence of this allele.