Your browser doesn't support javascript.
loading
n-3 Fatty acids preserve muscle mass and insulin sensitivity in a rat model of energy restriction.
Galmiche, Guillaume; Huneau, Jean-François; Mathé, Véronique; Mourot, Jacques; Simon, Noémie; Le Guillou, Céline; Hermier, Dominique.
Afiliação
  • Galmiche G; 1UMR Physiologie de la Nutrition et du Comportement Alimentaire,AgroParisTech,INRA,Université Paris-Saclay,75005 Paris,France.
  • Huneau JF; 1UMR Physiologie de la Nutrition et du Comportement Alimentaire,AgroParisTech,INRA,Université Paris-Saclay,75005 Paris,France.
  • Mathé V; 1UMR Physiologie de la Nutrition et du Comportement Alimentaire,AgroParisTech,INRA,Université Paris-Saclay,75005 Paris,France.
  • Mourot J; 2UMR PEGASE,Agrocampus Ouest,INRA,35590 Saint-Gilles,France.
  • Simon N; 3Terres Univia,11 rue de Monceau,CS 60003,75008 Paris,France.
  • Le Guillou C; 3Terres Univia,11 rue de Monceau,CS 60003,75008 Paris,France.
  • Hermier D; 1UMR Physiologie de la Nutrition et du Comportement Alimentaire,AgroParisTech,INRA,Université Paris-Saclay,75005 Paris,France.
Br J Nutr ; 116(7): 1141-1152, 2016 Oct.
Article em En | MEDLINE | ID: mdl-27619894
In obese subjects, the loss of fat mass during energy restriction is often accompanied by a loss of muscle mass. The hypothesis that n-3 PUFA, which modulate protein homoeostasis via effects on insulin sensitivity, could contribute to maintain muscle mass during energy restriction was tested in rats fed a high-fat diet (4 weeks) rich in 18 : 1 n-9 (oleic acid, OLE-R), 18 : 3 n-3 (α-linolenic acid, ALA-R) or n-3 long-chain (LC-R) fatty acid and then energy restricted (8 weeks). A control group (OLE-ad libitum (AL)) was maintained with AL diet throughout the study. Rats were killed 10 min after an i.v. insulin injection. All energy-restricted rats lost weight and fat mass, but only the OLE-R group showed a significant muscle loss. The Gastrocnemius muscle was enriched with ALA in the ALA-R group and with LC-PUFA in the ALA-R and LC-R groups. The proteolytic ubiquitin-proteasome system was differentially affected by energy restriction, with MAFbx and muscle ring finger-1 mRNA levels being decreased in the LC-R group (-30 and -20 %, respectively). RAC-α serine/threonine-protein kinase and insulin receptor substrate 1 phosphorylation levels increased in the LC-R group (+70 %), together with insulin receptor mRNA (+50 %). The ALA-R group showed the same overall activation pattern as the LC-R group, although to a lesser extent. In conclusion, dietary n-3 PUFA prevent the loss of muscle mass associated with energy restriction, probably by an improvement in the insulin-signalling pathway activation, in relation to enrichment of plasma membranes in n-3 LC-PUFA.
Assuntos
Palavras-chave
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Ácidos Graxos Ômega-3 / Músculo Esquelético / Restrição Calórica / Dieta Hiperlipídica Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Ácidos Graxos Ômega-3 / Músculo Esquelético / Restrição Calórica / Dieta Hiperlipídica Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article