[Interaction between functional nano-hydroxyapatite and cells and the underlying mechanisms].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 41(9): 937-45, 2016 Sep 28.
Article
em Zh
| MEDLINE
| ID: mdl-27640792
ABSTRACT
OBJECTIVE:
To explore the interaction between arginine functionalized hydroxyapatite (HAP/Arg) nanoparticles and endothelial cells, and to investigate mechanisms for endocytosis kinetics and endocytosis.â©METHODS:
Human umbilical vein endothelial cells (HUVECs) were selected as the research model.Cellular uptake of HAP/Arg nanoparticles were observed by laser scanning confocal microscopy.Average fluorescence intensity of cells after ingestion with different concentrations of HAP/Arg nanoparticles were determined by flow cytometer and atomic force microscopy.â©RESULTS:
The HAP/Arg nanoparticles with doped terbium existed in cytoplasm, and most of them distributed around the nucleus area after cellular uptake by HUVECs. Cellular uptake process of HAP/Arg nanoparticles in HUVECs was in a time and concentration dependent manner. 4 h and 50 mg/L was the best condition for uptake. HAP/Arg nanoparticles were easier to be up-taken into the cells than HAP nanoparticles without arginine functionalized.â©CONCLUSION:
HAP/Arg nanoparticles are internalized by HUVECs cells through an active transport and energy-dependent endocytosis process, and it is up-taken by cells mainly through caveolin-mediated endocytosis, but the clathrin-dependent endocytic pathway is also involved..
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Durapatita
/
Endocitose
/
Células Endoteliais da Veia Umbilical Humana
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article