Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification.
Methods Mol Biol
; 1459: 161-74, 2016.
Article
em En
| MEDLINE
| ID: mdl-27665558
ABSTRACT
This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vesículas Extracelulares
/
Proteínas de Choque Térmico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article