Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
Cell
; 167(2): 471-483.e10, 2016 Oct 06.
Article
em En
| MEDLINE
| ID: mdl-27693358
ABSTRACT
Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembly. These findings establish a mammalian translational plasticity pathway in mitochondria that enables adaptation of mitochondrial protein synthesis to the influx of nuclear-encoded subunits.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo IV da Cadeia de Transporte de Elétrons
/
Proteínas Mitocondriais
/
Ciclo-Oxigenase 1
/
Proteínas de Membrana
/
Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article