Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages.
Dev Growth Differ
; 58(9): 679-687, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27759163
ABSTRACT
In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta-actin promoter. The aNLS-EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution. Furthermore, male and female pronuclei were visualized in fertilized eggs, and nuclear dynamics of pronuclear fusion and subsequent cleavage were captured by time-lapse imaging. In contrast, SV40NLS exhibited no activity of nuclear transport in early embryos. In conclusion, the aNLS possesses a strong nuclear localization activity and is a useful probe for fluorescent observation of the pronuclei and nuclei in early developmental stage of medaka.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryzias
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Animais Geneticamente Modificados
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Núcleo Celular
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Proteínas de Fluorescência Verde
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article