High-Throughput Discovery of Aptamers for Sandwich Assays.
Anal Chem
; 88(22): 10842-10847, 2016 11 15.
Article
em En
| MEDLINE
| ID: mdl-27813404
ABSTRACT
Sandwich assays are among the most powerful tools in molecular detection. These assays use "pairs" of affinity reagents so that the detection signal is generated only when both reagents bind simultaneously to different sites on the target molecule, enabling highly sensitive and specific measurements in complex samples. Thus, the capability to efficiently screen affinity reagent pairs at a high throughput is critical. In this work, we describe an experimental strategy for screening "aptamer pairs" at a throughput of 106 aptamer pairs per hour-which is many orders of magnitude higher than the current state of the art. The key step in our process is the conversion of solution-phase aptamers into "aptamer particles" such that we can directly measure the simultaneous binding of multiple aptamers to a target protein based on fluorescence signals and sort individual particles harboring aptamer pairs via the fluorescence-activated cell-sorter instrument. As proof of principle, we successfully isolated a high-quality DNA aptamer pair for plasminogen activator inhibitor 1 (PAI-1). Within only two rounds of screening, we discovered DNA aptamer pairs with low-nanomolar sensitivity in dilute serum and excellent specificity with minimal off-target binding even to closely related proteins such as PAI-2.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Aptâmeros de Nucleotídeos
/
Ensaios de Triagem em Larga Escala
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article