Isolation of Type III Secretion System Needle Complexes by Shearing.

Nilles, Matthew L; Condry, Danielle L Jessen; Osei-Owusu, Patrick

Nilles, Matthew L; Condry, Danielle L Jessen; Osei-Owusu, Patrick.

Afiliação

Nilles ML; Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
Condry DL; Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
Osei-Owusu P; Department of Microbiology, University of Chicago, 920 East 59th St., Chicago, IL, 60637, USA. poseiowusu@uchicago.edu.

Methods Mol Biol ; 1531: 61-70, 2017.

Article em En | MEDLINE | ID: mdl-27837481

Assuntos

Proteínas de Bactérias/metabolismo; Bactérias Gram-Negativas/metabolismo; Complexos Multiproteicos/isolamento & purificação; Complexos Multiproteicos/metabolismo; Sistemas de Secreção Tipo III/metabolismo; Western Blotting; Eletroforese

Palavras-chave

Needle complex; Purification of needle proteins; Translocon; Type III secretion needle proteins; Type III secretion system

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Complexos Multiproteicos / Sistemas de Secreção Tipo III / Bactérias Gram-Negativas Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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