Advancement of green process through microwave-assisted extraction of bioactive metabolites from Arthrospira Platensis and bioactivity evaluation.
Bioresour Technol
; 224: 618-629, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27838319
ABSTRACT
Bioactivity and functional properties of cyanobacterial extract mostly depends on process of extraction, temperature and solvent used (polar or non-polar). To evaluate these parameters a design of experiment (DOE; using a 2k design) was performed with Arthrospira platensis. Extraction process was optimized through microwave-assisted extraction considering solvent ratio, temperature and time of extraction with polar (PS) and non-polar (NPS). Maximum extract yield obtained was 4.32±0.25% and 5.26±0.11% (w/w) respectively for PS and NPS. Maximum content of bioactive metabolites in PS extracts were thiamine (846.57±14.12µg/g), riboflavin (101.09±1.63µg/g), C-phycocyanin (2.28±0.10µg/g) and A-phycocyanin (4.11±0.03µg/g), while for NPS extracts were α-tocopherol (37.86±0.78µg/g), ß-carotene (123.64±1.45µg/g) and 19.44±0.21mg/g of fatty acids. A. platensis PS extracts showed high antimicrobial activity and PS extracts had antioxidant activity of 0.79±0.12µmolTE/g for FRAP assay, while for NPS extracts 1.03±0.08µmol α-TE/g for FRAP assay.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Spirulina
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Fracionamento Químico
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Micro-Ondas
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article