Evidence for dual receptor-binding sites in Clostridium difficile toxin A.
FEBS Lett
; 590(24): 4550-4563, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27861794
ABSTRACT
TcdA (308 kDa) and TcdB (270 kDa) disrupt the integrity of the intestinal epithelial barrier and provide an environment favorable for Clostridium difficile colonization. Recent evidence suggests that entry of TcdA into cells is mediated by at least two domains. Here, we report the characterization of a second receptor-binding domain (RBD2) for TcdA. While both the isolated combined repetitive oligopeptides (CROPs) and RBD2 fragments are rapidly internalized into cells under physiologic conditions, only the CROPs domain appreciably accumulates at the cell surface. Once internalized, CROPs and RBD2 are trafficked to late endosomal compartments. An internal deletion of RBD2 from TcdA holotoxin ablated toxicity in HT29 cells. These data are consistent with the recently proposed dual receptor model of cellular entry.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Membrana Celular
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Clostridioides difficile
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Enterotoxinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article