Molecular basis of thermostability enhancement of Renilla luciferase at higher temperatures by insertion of a disulfide bridge into the structure.

Fanaei Kahrani, Zahra; Emamzadeh, Rahman; Nazari, Mahboobeh; Rasa, Seyed Mohammad Mahdi

Fanaei Kahrani, Zahra; Emamzadeh, Rahman; Nazari, Mahboobeh; Rasa, Seyed Mohammad Mahdi.

Afiliação

Fanaei Kahrani Z; Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Emamzadeh R; Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.. Electronic address: Sci_rahman@yahoo.com.
Nazari M; Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Rasa SMM; Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.

Biochim Biophys Acta Proteins Proteom ; 1865(2): 252-259, 2017 Feb.

Article em En | MEDLINE | ID: mdl-27863256

Assuntos

Dissulfetos/química; Dissulfetos/metabolismo; Luciferases de Renilla/química; Luciferases de Renilla/metabolismo; Biotecnologia/métodos; Domínio Catalítico/fisiologia; Estabilidade Enzimática/fisiologia; Temperatura Alta; Cinética; Luz; Simulação de Dinâmica Molecular; Mutagênese Sítio-Dirigida/métodos; Temperatura

Palavras-chave

Disulfide bridge; Kinetic stability; Molecular dynamic simulation; Super Renilla luciferase 8 (SRLuc 8)

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferases de Renilla / Dissulfetos Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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