Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography.
Anal Biochem
; 517: 53-55, 2017 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-27865825
ABSTRACT
The paper reports an inexpensive and efficient procedure for the removal of protein S1 from E. coli ribosomes. It comprises incubation of ribosomes in a pyrimidine polyribonucleotide solution followed by centrifugation of the sample through a sucrose cushion. To avoid co-sedimentation of the S1-bound polypyrimidine with the ribosomes, its length should not exceed several hundred nucleotides. Unlike popular affinity chromatography through a poly(U) Sepharose or poly(U) cellulose column, the method tolerates limited polyribonucleotide degradation by eventual traces of ribonucleases, and can readily be incorporated into standard protocols for the isolation of ribosomes by centrifugation.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
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Ribossomos
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Proteínas de Escherichia coli
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Escherichia coli
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article