Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond.

A Gram-stain-negative, non-motile, yellow-pigmented bacterium, designated strain IMCC27201T, was isolated from an artificial freshwater pond (Inkyong) in Korea. Growth of strain IMCC27201T occurred at 10-37 °C (optimum, 30 °C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-0.25 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC27201T belonged to the genus Flavobacterium and was related most closely to Flavobacterium columnare IFO 15943T (97.8 % sequence similarity), Flavobacterium terrae R2A1-13T (97.2 %) and Flavobacterium vireti THG-SM1T (96.4 %). Strain IMCC27201T exhibited low DNA-DNA relatedness with F. columnare KCTC 42885T (34.9-40.6 %). The DNA G+C content of strain IMCC27201T was 32.1 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C15 : 0 3-OH. Strain IMCC27201T contained menaquinone-6 (MK-6) as the sole isoprenoid quinone. The polar lipids of strain IMCC27201T consisted of phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene phylogeny, phenotypic characterization and DNA-DNA relatedness, strain IMCC27201Trepresents a novel species of the genus Flavobacterium, for which the name Flavobacterium inkyongense sp. nov. is proposed. The type strain is IMCC27201T (=KCTC 52244T=JCM 31385T).