Assessing translational efficiency by a reporter protein co-expressed in a cell-free synthesis system.

Park, Yu Jin; Lee, Kyung-Ho; Kim, Dong-Myung

Park, Yu Jin; Lee, Kyung-Ho; Kim, Dong-Myung.

Afiliação

Park YJ; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
Lee KH; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
Kim DM; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea. Electronic address: dmkim@cnu.ac.kr.

Anal Biochem ; 518: 139-142, 2017 Feb 01.

Article em En | MEDLINE | ID: mdl-27908596

Assuntos

Escherichia coli/química; Genes Reporter; Proteínas de Fluorescência Verde/biossíntese; Biossíntese de Proteínas; Sistema Livre de Células/química; Sistema Livre de Células/metabolismo; Escherichia coli/metabolismo; Proteínas de Fluorescência Verde/química

Palavras-chave

Assessment of gene expression; Cell-free protein synthesis; Co-expression; Translational efficiency

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / Genes Reporter / Proteínas de Fluorescência Verde / Escherichia coli Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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