LC-MS-MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves.
Pharm Biol
; 55(1): 88-95, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-27925471
ABSTRACT
CONTEXT Despite some studies related to Juniperus phoenicea L. (Cupressaceae), phytochemical and biological investigations of this plant remain unexplored. OBJECTIVE:
This work is the first report dealing with the identification and characterization of volatile components and flavonoids in hexane and methanol extracts from J. phoenicea leaves Materials andmethods:
Antioxidant activity of hexane, and methanol extracts from J. phoenicea leaves were determined by DPPH-radical scavenging assay. α-Amylase inhibitory activity was evaluated by enzyme inhibition using in vitro assay (each extract was dissolved in DMSO to give concentrations of 50, 100 and 200 mg/mL). The chemical composition of fractions (Fr1-Fr3) from methanol extract was determined by high-performance liquid chromatography coupled with mass spectroscopy (HPLC-MS) analysis. RESULTS ANDDISCUSSION:
The hexane extract was analyzed by GC-MS technique which allowed the identification of 32 compounds. The main constituents were α-humulene (16.9%), pentadecane (10.2%) and α-cubebene (9.7%). Fraction Fr 2 exhibited a strong DPPH radical-scavenging activity (IC50 = 20.1 µg/mL) compared to that of BHT as well as the highest α-amylase inhibitory activity (IC50 = 28.4 µg/mL). Three flavonoids were identified in these fractions using HPLC-MSanalysis:
Quercetin 3-O-glucoside, isoscutellarein 7-O-pentoside and quercetin 3-O-pentoside. In addition, the more active fraction (Fr 2) was purified with semi-preparative HPLC affording one pure compound (amentoflavone) using 1H NMR analysis. This compound exhibited powerful DPPH radical-scavenging (IC50 = 14.1 µg/mL) and α-amylase inhibition (IC50 = 20.4 µg/mL) effects.CONCLUSION:
This study provides scientific support to some medicinal uses of J. phoenicea found in North Africa.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Extratos Vegetais
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Cromatografia Líquida de Alta Pressão
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Folhas de Planta
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Juniperus
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Espectrometria de Massas em Tandem
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Compostos Orgânicos Voláteis
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Cromatografia Gasosa-Espectrometria de Massas
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article