Incorporation of an Unnatural Amino Acid as a Domain-Specific Fluorescence Probe in a Two-Domain Protein.
Biochemistry
; 55(49): 6739-6742, 2016 Dec 13.
Article
em En
| MEDLINE
| ID: mdl-27951650
ABSTRACT
The biophysical analysis of multidomain proteins often is difficult because of overlapping signals from the individual domains. Previously, the fluorescent unnatural amino acid p-cyanophenylalanine has been used to study the folding of small single-domain proteins. Here we extend its use to a two-domain protein to selectively analyze the folding of a specific domain within a multidomain protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Corantes Fluorescentes
/
Aminoácidos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article