Antiaging effects of melatonin on the myocardial mitochondria of rats and associated mechanisms.
Mol Med Rep
; 15(1): 403-410, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27959405
ABSTRACT
The present study investigated the anti-aging effects of melatonin on the myocardial mitochondria of D-galactose-aged rats and associated mechanisms. A total of 30 male SpragueDawley (SD) rats were randomly divided into three equal groups An accelerated aging group that received 125 mg/kg/day Dgalactose; a melatonintreated group of Dgalactoseaged rats that received 10 mg/kg/day melatonin; and a control group receiving normal saline. ATP, ADP and AMP levels in the left ventricular myocardium of rats were determined by high performance liquid chromatography and the total adenylic acid number (TAN) was subsequently calculated. Bax, Bcl2, and cytochrome c (cytc) protein expression levels in myocardial mitochondria and cytoplasm were quantified by western blot analysis. In the melatonintreated group, ATP levels were significantly higher when compared with the untreated control group and the acceleratedageing group (0.068 vs. 0.052 and 0.058; P=0.002 and P=0.045, respectively), and TAN was significantly increased in the melatonintreated group when compared with controls (P=0.011). In addition, cytc levels in the cytoplasm, but not in the mitochondria, were significantly higher in the acceleratedaging group compared with the control and melatonintreated groups (P=0.001 and P=0.002, respectively). Bcl2 and Bax ratios were significantly higher in the control and melatonintreated groups when compared with the acceleratedaging group (P=0.004 and P=0.032, respectively). These results suggest that melatonin exhibits a protective effect on mitochondrial function in a rat model of accelerated aging.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Envelhecimento
/
Melatonina
/
Mitocôndrias Cardíacas
/
Antioxidantes
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article