cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.
Nat Methods
; 14(2): 145-148, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27992408
ABSTRACT
The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Engenharia Genética
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Regulação da Expressão Gênica
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Caenorhabditis elegans
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article