Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection.
G3 (Bethesda)
; 7(2): 719-722, 2017 02 09.
Article
em En
| MEDLINE
| ID: mdl-28040780
ABSTRACT
Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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RNA Guia de Cinetoplastídeos
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Marcação de Genes
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Endonucleases
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Edição de Genes
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article