Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.
Nat Methods
; 14(2): 141-144, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-28068317
ABSTRACT
We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óptica e Fotônica
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Corantes Fluorescentes
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Histidina Quinase
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Imagem Individual de Molécula
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Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article