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Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation.
Kanamori, Takashi; Takamura, Akihiro; Tago, Nobuhiro; Masaki, Yoshiaki; Ohkubo, Akihiro; Sekine, Mitsuo; Seio, Kohji.
Afiliação
  • Kanamori T; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Takamura A; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Tago N; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Masaki Y; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Ohkubo A; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Sekine M; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
  • Seio K; Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-ku, Yokohama 226-8501, Japan. msekine@bio.titech.ac.jp kseio@bio.titech.ac.jp.
Org Biomol Chem ; 15(5): 1190-1197, 2017 Feb 01.
Article em En | MEDLINE | ID: mdl-28084483
ABSTRACT
Green fluorescent protein (GFP)-based molecular-rotor chromophores were attached to the 5-positions of deoxyuridines, and subsequently, incorporated into the middle positions of oligodeoxynucleotides. These oligonucleotides were designed to form triplex DNA in order to encapsulate the GFP chromophores, mimicking GFP structures. Upon triplex formation, the embedded GFP chromophores exhibited fluorescence enhancement, suggesting the potential application of these fluorescent probes for the detection of nucleic acids.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligodesoxirribonucleotídeos / DNA / Proteínas de Fluorescência Verde / Fluorescência Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligodesoxirribonucleotídeos / DNA / Proteínas de Fluorescência Verde / Fluorescência Idioma: En Ano de publicação: 2017 Tipo de documento: Article