Development of Clostridium difficile R20291&#916;PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.

Bilverstone, T W; Kinsmore, N L; Minton, N P; Kuehne, S A

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.

Bilverstone, T W; Kinsmore, N L; Minton, N P; Kuehne, S A.

Afiliação

Bilverstone TW; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
Kinsmore NL; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS
Minton NP; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS
Kuehne SA; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK; NIHR Nottingham Digestive Diseases, (NDCC) Biomedical Research Unit, Nottingham University Hospitals NHS

Anaerobe ; 44: 51-54, 2017 Apr.

Article em En | MEDLINE | ID: mdl-28108389

ABSTRACT

ABSTRACT

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Assuntos

ADP Ribose Transferases/metabolismo; Proteínas de Bactérias/metabolismo; Clostridioides difficile/genética; Clostridioides difficile/metabolismo; Deleção de Genes; Regulação Bacteriana da Expressão Gênica; Genes Reguladores; ADP Ribose Transferases/toxicidade; Animais; Proteínas de Bactérias/toxicidade; Sobrevivência Celular/efeitos dos fármacos; Chlorocebus aethiops; Células Vero

Palavras-chave

Binary toxin; C. difficile; CdtR; PaLoc; Virulence

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Genes Reguladores / Clostridioides difficile / ADP Ribose Transferases / Deleção de Genes Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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