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Endothelial Basement Membrane Laminin 511 Contributes to Endothelial Junctional Tightness and Thereby Inhibits Leukocyte Transmigration.
Song, Jian; Zhang, Xueli; Buscher, Konrad; Wang, Ying; Wang, Huiyu; Di Russo, Jacopo; Li, Lixia; Lütke-Enking, Stefan; Zarbock, Alexander; Stadtmann, Anika; Striewski, Paul; Wirth, Benedikt; Kuzmanov, Ivan; Wiendl, Heinz; Schulte, Dörte; Vestweber, Dietmar; Sorokin, Lydia.
Afiliação
  • Song J; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Zhang X; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Buscher K; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Wang Y; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Wang H; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Di Russo J; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Li L; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Lütke-Enking S; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany.
  • Zarbock A; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Department of Anesthesiology and Intensive Care Medicine, University of Muenster, 48149 Muenster, Germany.
  • Stadtmann A; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Department of Anesthesiology and Intensive Care Medicine, University of Muenster, 48149 Muenster, Germany.
  • Striewski P; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Institute for Computational and Applied Mathematics, University of Muenster, 48149 Muenster, Germany.
  • Wirth B; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Institute for Computational and Applied Mathematics, University of Muenster, 48149 Muenster, Germany.
  • Kuzmanov I; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Department of Neurology, University Hospital of Muenster, University of Muenster, 48149 Muenster, Germany.
  • Wiendl H; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Department of Neurology, University Hospital of Muenster, University of Muenster, 48149 Muenster, Germany.
  • Schulte D; Max-Planck Institute of Molecular Biomedicine, 48149 Muenster, Germany.
  • Vestweber D; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany; Max-Planck Institute of Molecular Biomedicine, 48149 Muenster, Germany.
  • Sorokin L; Institute of Physiological Chemistry and Pathobiochemistry, University of Muenster, 48149 Muenster, Germany; Cells-in-Motion Cluster of Excellence, University of Muenster, 48149 Muenster, Germany. Electronic address: sorokin@uni-muenster.de.
Cell Rep ; 18(5): 1256-1269, 2017 01 31.
Article em En | MEDLINE | ID: mdl-28147279
ABSTRACT
Endothelial basement membranes constitute barriers to extravasating leukocytes during inflammation, a process where laminin isoforms define sites of leukocyte exit; however, how this occurs is poorly understood. In addition to a direct effect on leukocyte transmigration, we show that laminin 511 affects endothelial barrier function by stabilizing VE-cadherin at junctions and downregulating expression of CD99L2, correlating with reduced neutrophil extravasation. Binding of endothelial cells to laminin 511, but not laminin 411 or non-endothelial laminin 111, enhanced transendothelial cell electrical resistance (TEER) and inhibited neutrophil transmigration. Data suggest that endothelial adhesion to laminin 511 via ß1 and ß3 integrins mediates RhoA-induced VE-cadherin localization to cell-cell borders, and while CD99L2 downregulation requires integrin ß1, it is RhoA-independent. Our data demonstrate that molecular information provided by basement membrane laminin 511 affects leukocyte extravasation both directly and indirectly by modulating endothelial barrier properties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membrana Basal / Endotélio Vascular / Movimento Celular / Laminina / Células Endoteliais / Leucócitos Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membrana Basal / Endotélio Vascular / Movimento Celular / Laminina / Células Endoteliais / Leucócitos Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article