Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production.
Glycobiology
; 27(5): 392-399, 2017 05 01.
Article
em En
| MEDLINE
| ID: mdl-28168306
ABSTRACT
It was recently shown that Mycobacterium tuberculosis produces cellulose which forms an integral part of its extracellular polymeric substances within a biofilm set-up. Using Mycobacterium smegmatis as a proxy model organism, we demonstrate that M. smegmatis biofilms treated with purified MSMEG_6752 releases the main cellulose degradation-product (cellobiose), detected by using ionic chromatography, suggesting that MSMEG_6752 encodes a cellulase. Its overexpression in M. smegmatis prevents spontaneous biofilm formation. Moreover, the method reported here allowed detecting cellobiose when M. smegmatis cultures were exposed to a subinhibitory dose of rifampicin. Overall, this study highlights the role of the MSMEG_6752 in managing cellulose production induced during biofilm formation and antibiotic stress response.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Celulase
/
Celulose
/
Biofilmes
/
Mycobacterium smegmatis
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article