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Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?
Verner-Jeffreys, David W; Brazier, Thomas; Perez, Ramon Y; Ryder, David; Card, Roderick M; Welch, Timothy J; Hoare, Rowena; Ngo, Thao; McLaren, Nikki; Ellis, Richard; Bartie, Kerry L; Feist, Stephen W; Rowe, William M P; Adams, Alexandra; Thompson, Kim D.
Afiliação
  • Verner-Jeffreys DW; Cefas Weymouth laboratory, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.
  • Brazier T; Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK.
  • Perez RY; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • Ryder D; Cefas Weymouth laboratory, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.
  • Card RM; APHA Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Welch TJ; USDA/ARS National Center for Cool and Cold Water Aquaculture, 11861 Leetown Rd., Kearneysville WV 25430, USA.
  • Hoare R; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • Ngo T; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • McLaren N; APHA Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Ellis R; APHA Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
  • Bartie KL; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • Feist SW; Cefas Weymouth laboratory, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.
  • Rowe WMP; Institute of Integrative Biology, University of Liverpool, Merseyside L69 3BX, UK.
  • Adams A; Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
  • Thompson KD; Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
FEMS Microbiol Ecol ; 93(4)2017 04 01.
Article em En | MEDLINE | ID: mdl-28199699
ABSTRACT
Bacteria from the family Flavobacteriaceae often show low susceptibility to antibiotics. With the exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates from salmonid fish that could grow in ≥ 4 mg/L florfenicol. Whole genome sequence analysis of the floR positive isolates revealed the presence of a region that contained the antimicrobial resistance genes floR, a tet(X) tetracycline resistance gene, a streptothricin resistance gene and a chloramphenicol acetyltransferase gene. In silico analysis of 377 published genomes for Flavobacteriaceae isolates from a range of sources confirmed that well-characterised resistance gene cassettes were not widely distributed in bacteria from this group. Efflux pump-mediated decreased susceptibility to a range of antimicrobials was confirmed in both floR positive isolates using an efflux pump inhibitor (phenylalanine-arginine ß-naphthylamide) assay. The floR isolates possessed putative virulence factors, including production of siderophores and haemolysins, and were mildly pathogenic in rainbow trout. Results support the suggestion that, despite the detection of floR, susceptibility to antimicrobials in Flavobacteriaceae is mostly mediated via intrinsic mechanisms rather than the horizontally acquired resistance genes more normally associated with Gram-negative bacterial pathogens such as Enterobacteriaceae.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tianfenicol / Proteínas de Bactérias / Oncorhynchus mykiss / Chryseobacterium / Antibacterianos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tianfenicol / Proteínas de Bactérias / Oncorhynchus mykiss / Chryseobacterium / Antibacterianos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article