Detecting DNA cytosine methylation using nanopore sequencing.
Nat Methods
; 14(4): 407-410, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28218898
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Humano
/
Metilação de DNA
/
Citosina
/
5-Metilcitosina
Tipo de estudo:
Health_economic_evaluation
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article