Studies on the immunoglobulin V kappa locus in human lymphoid cell lines.

DNA digests of 16 human lymphoid cell lines were studied in blot hybridization experiments with probes from V kappa genes and their immediate neighborhood as well as with single or low-copy probes from intergenic regions. The patterns were compared with those of placenta DNA digests in which the kappa genes are in the germline configuration. The differences of patterns which were detected with the first type of hybridization probes can be attributed to V kappa--J kappa rearrangements or to restriction site polymorphisms between individuals. Some of the pattern differences observed with the second type of probes can be interpreted best as arising from deletions of parts of the kappa locus. Such deletions may be individual variations but they may also be caused by the V kappa-J kappa rearrangement process. The results obtained with one particular probe which was derived from a nonduplicated part of the kappa locus allow some conclusions as to the mechanism of the V kappa--J kappa rearrangement: the genomic situation in some lymphoid cell lines can be explained by an inversion while in other cell lines clearly deletions have occurred. The observations are in agreement with the inversion-deletion mechanism of V kappa--J kappa rearrangement as proposed by Lewis et al. (1982, 1984).