Effect of phenobarbital on metabolism of polyphosphoinositides of rat brain synaptosomes.
Life Sci
; 41(18): 2121-6, 1987 Nov 02.
Article
em En
| MEDLINE
| ID: mdl-2823040
ABSTRACT
Synthesis and degradation of polyphosphoinositides in a rat brain synaptosome preparation were depressed by phenobarbital. Phosphatidylinositol-4-phosphate kinase (PIP-kinase), the enzyme which synthesizes phosphatidylinositol-4,5-bisphosphate (PIP2) was most strongly affected (50% inhibition at 3 mM phenobarbital); phosphatidylinositol (PI-kinase) followed (50% at 15 mM). The phosphoesterases were less sensitive PIP-monoesterase (50% at 39 mM), PIP2-monoesterase (at 47 mM), and, least inhibited, PIP-diesterase (50% at 65 mM) and PIP2-diesterase (at 68 mM). Phenobarbital by inhibiting PIP-kinase may reduce the membrane concentration of PIP2 and thus dampen the stimulus-response which leads to the hydrolysis of PIP2 and the formation of the second messenger, inositol-1,4,5-trisphosphate (IP3), involved in mobilization of intracellular Ca2+.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenobarbital
/
Fosfatidilinositóis
/
Sinaptossomos
/
Encéfalo
/
Fosfotransferases (Aceptor do Grupo Álcool)
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article