Increased transforming growth factor ß and interleukin 10 transcripts in peripheral blood mononuclear cells of colorectal cancer patients.

AIM OF THE STUDY: The ability of immune cells in peripheral blood to produce certain cytokines affects tumour-elicited inflammation. The aim of this study was to investigate the gene expression of interleukin 12A (IL-12A), IL-12B, IL-23A, IL-10, IL-6, transforming growth factor ß (TGF-ß), HDAC3, and iNOS in peripheral blood mononuclear cells (PBMC) from colorectal cancer (CRC) patients. MATERIAL AND METHODS: The venous blood for PBMC isolation was collected preoperatively and 10 days after surgery, from CRC patients. After isolation of total RNA and synthesis of cDNA, quantitative real-time PCR assays were performed. RESULTS: Our results demonstrated that among investigated cytokine genes IL-10 and TGF-ß were significantly upregulated in patients with CRC compared to the control group, while the expression of IL-23 mRNA was significantly decreased in CRC patients. We observed significantly increased mRNA levels in CRC patients' PBMC before surgery for IL-10 and TGF-ß compared to both postoperative and control groups. We also found a significant upregulation of iNOS in early compared to advanced CRC. CONCLUSIONS: Based on the results we can assume that PBMC gene expression programming in CRC patients drives local differentiation of Th cells towards Treg instead of the Th1 anti-tumour subpopulation.