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Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells.
Fan, Yanlin; Guo, Yusong R; Yuan, Wang; Zhou, Ying; Holt, Matthew V; Wang, Tao; Demeler, Borries; Young, Nicolas L; Zhong, Weiwei; Tao, Yizhi J.
Afiliação
  • Fan Y; Department of BioSciences, Rice University, MS-140, Houston, Texas, United States of America.
  • Guo YR; Department of BioSciences, Rice University, MS-140, Houston, Texas, United States of America.
  • Yuan W; Department of BioSciences, Rice University, MS-140, Houston, Texas, United States of America.
  • Zhou Y; Department of BioSciences, Rice University, MS-140, Houston, Texas, United States of America.
  • Holt MV; Verna & Marrs McLean Department of Biochemistry & Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, United States of America.
  • Wang T; Verna & Marrs McLean Department of Biochemistry & Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, United States of America.
  • Demeler B; The University of Texas Health Science Center at San Antonio, Department of Biochemistry, MC 7760, 7703 Floyd Curl Drive, San Antonio, Texas, United States of America.
  • Young NL; Verna & Marrs McLean Department of Biochemistry & Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, United States of America.
  • Zhong W; Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, United States of America.
  • Tao YJ; Department of BioSciences, Rice University, MS-140, Houston, Texas, United States of America.
PLoS Pathog ; 13(2): e1006231, 2017 02.
Article em En | MEDLINE | ID: mdl-28241071
Despite the wide use of Caenorhabditis elegans as a model organism, the first virus naturally infecting this organism was not discovered until six years ago. The Orsay virus and its related nematode viruses have a positive-sense RNA genome, encoding three proteins: CP, RdRP, and a novel δ protein that shares no homology with any other proteins. δ can be expressed either as a free δ or a CP-δ fusion protein by ribosomal frameshift, but the structure and function of both δ and CP-δ remain unknown. Using a combination of electron microscopy, X-ray crystallography, computational and biophysical analyses, here we show that the Orsay δ protein forms a ~420-Å long, pentameric fiber with an N-terminal α-helical bundle, a ß-stranded filament in the middle, and a C-terminal head domain. The pentameric nature of the δ fiber has been independently confirmed by both mass spectrometry and analytical ultracentrifugation. Recombinant Orsay capsid containing CP-δ shows protruding long fibers with globular heads at the distal end. Mutant viruses with disrupted CP-δ fibers were generated by organism-based reverse genetics. These viruses were found to be either non-viable or with poor infectivity according to phenotypic and qRT-PCR analyses. Furthermore, addition of purified δ proteins to worm culture greatly reduced Orsay infectivity in a sequence-specific manner. Based on the structure resemblance between the Orsay CP-δ fiber and the fibers from reovirus and adenovirus, we propose that CP-δ functions as a cell attachment protein to mediate Orsay entry into worm intestine cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / Caenorhabditis elegans / Proteínas do Capsídeo / Internalização do Vírus Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / Caenorhabditis elegans / Proteínas do Capsídeo / Internalização do Vírus Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2017 Tipo de documento: Article