Induction of chromosomal aberrations in Chinese hamster ovary cells by the restriction endonuclease Dra I: dose-effect relationships and effect of substitution of chromosomal DNA with bromodeoxyuridine.
Mutat Res
; 192(4): 263-9, 1987 Dec.
Article
em En
| MEDLINE
| ID: mdl-2825011
ABSTRACT
Treatment of G1-phase Chinese hamster ovary (CHO) cells with the restriction endonuclease Dra I (recognition site TTT/AAA) leads to the induction of chromosome-type aberrations. The dose-effect relationships or the frequencies of polycentric chromosomes have a strong linear component. Prelabelling of the cells with bromodeoxyuridine (B) leads to a strong suppression by the chromosome breaking activity of Dra I. This may be explained by assuming that substitution of T by B renders the recognition site of Dra I resistant to being cut by the enzyme.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bromodesoxiuridina
/
DNA
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
Enzimas de Restrição do DNA
/
Aberrações Cromossômicas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article